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Post Info TOPIC: Corneal Stroma Cell Density Evolution in Keratoconus Corneas Following the Implantation of Adipose Mesenchymal Stem Cell


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Date: Sun Apr 26 6:25 AM, 2020
Corneal Stroma Cell Density Evolution in Keratoconus Corneas Following the Implantation of Adipose Mesenchymal Stem Cell
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Corneal Stroma Cell Density Evolution in Keratoconus Corneas Following the Implantation of Adipose Mesenchymal Stem Cells and Corneal Laminas: An In Vivo Confocal Microscopy Study

Mona El Zarif; Karim A. Jawad; Jorge L. Alió Del Barrio; Ziad A. Jawad; Antonio Palazón-Bru; María P. de Miguel; Peggy Saba; Nehman Makdissy; Jorge L. Alió

 Author Affiliations & Notes

Investigative Ophthalmology & Visual Science April 2020, Vol.61, 22. doi:https://doi.org/10.1167/iovs.61.4.22

 

Purpose: To report the corneal stroma cell density evolution identified by in vivo corneal confocal microscopy in humans using injected autologous adipose-derived adult stem cells (ADASCs) and corneal decellularized laminas in corneas with advanced keratoconus.

Methods: Interventional prospective, consecutive, randomized, comparative series of cases. A total of 14 keratoconic patients were randomly distributed into three groups for three types of surgical interventions: group 1 (G-1), autologous ADASC implantation (n = 5); group 2 (G-2), decellularized human corneal stroma (n = 5); and group 3 (G-3), autologous ADASCs + decellularized human corneal stroma (n = 4).

Results: A gradual and significant increase (P < 0.001) was observed in the cellularity in the anterior and posterior stroma of patients in G-1, G-2, and G-3 a year after the surgery in comparison with the preoperative density level. The same result was observed at the mid-corneal stroma in G-1 and at the anterior and posterior surfaces and within the laminas in G-2 and G-3. The cell density of patients receiving ADASC recellularized laminas (G-3) was statistically significantly higher (P = 0.011) at the anterior surface and within the lamina (P = 0.029) and at the posterior surface than in those implanted only with decellularized laminas (G-2).

Conclusions: A significant increase in cell density occurred up to 1 postoperative year at the corneal stroma following the implantation of ADASCs alone, as well as in those cases implanted with decellularized and recellularized laminas at the different levels of the analysis. However, this increase was significantly higher in the ADASC recellularized laminas.



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Date: Sun Apr 26 6:45 AM, 2020
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Nice to see that matters are moving in the right direction!

By the way:

In an in vivo (latin: "in the living") test, is a study that is performed in living organisms (i.e, rat, rabbit).

Cell density refers to the number of cells per unit volume. Often cell density is denoted as viable cell density which is the number of living cells per unit volume.



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